Figure 7: Tbr1^layer6 mutants exhibit increased aggressive and anxiety-like behavior at P56P80.

Behavioral analysis of Tbr1^wildtype (red), Tbr1^layer6 heterozygous (green), and Tbr1^layer6 homozygous mutants (blue). (A) Rotarod assay did not demonstrate any impaired movement or motor coordination in Tbr1^layer6 mutants. (B) Tbr1^layer6 heterozygous (green) mutants spent more time in the closed arm of the elevated plus maze (an anxiety-like phenotype) compared to their Tbr1^wildtype littermates. (C) Loss of Tbr1 did not affect the time spent engaged in novel object exploration or (D) social interactions. (D) Tbr1^layer6 homozygous mutants (blue) exhibited aggressive behaviors when interacting with a novel juvenile mouse. Two-tailed T-test with tukey correction was used for pairwise comparisons (*p<0.05) (***p<0.001).