a,Total ROS production following treatment of bak1–4 mesophyll protoplasts with 100 nM elf18 over 30 min. The total relative luminescence unit (RLU) values from n=4 independent biological experiments are normalized relative to protoplasts expressing wild-type (WT) BAK1 and expressed as aligned dot blots showing mean ± SE. b, Total ROS production following treatment with 100 nM elf18 over 60 min of n=8 biologically independent suspensions of bak1–4 mesophyll protoplasts transiently expressing the indicated BAK1 mutants,. Measurements are plotted as boxplots displaying the first and third quartiles, split by the median; whiskers extend to a maximum of 1.5 × interquartile range beyond the box. Circles indicate individual data points. c, Western blot analysis with α-BAK1 antibodies. For blot source data, see Supplementary Figure 1. a-b, Statistical analysis was performed using one-way ANOVA and Dunnett’s post-hoc test compared to the WT control. b,c, Experiments were repeated twice with similar results.