Figure 1 |. Identification of BAK1 phosphosites.

a, Representative Coomasie brilliant Blue (CBB)-stained SDS-PAGE gel showing proteins enriched upon GFP immune-precipitation. b, Western blot analysis of BAK1 co-immunoprecipitated with FLS2-GFP and EFR-GFP proteins from (a) using α-GFP and α-BAK1 antibodies. a, b, Experiments were independently repeated three times. For gel and blot source data, see Supplementary Figure 1. c, Summary of BAK1 in vivo phosphosites identified by FLS2-GFP and EFR-GFP co-immunoprecipitation followed by LC-MS/MS analysis. Spectra of identified sites are presented in Supplementary Figure 2.