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. Author manuscript; available in PMC: 2019 Mar 3.
Published in final edited form as: Nature. 2018 Sep 3;561(7722):248–252. doi: 10.1038/s41586-018-0471-x

Figure 2 |. Individual or double BAK1-S602A, -T603A, or -S604A mutations do not affect elf18-induced ROS production.

Figure 2 |

a, e, Total ROS production following treatment with 100 nM elf18 over 60 min of n=12 biological independent suspensions of bak1–4 mesophyll protoplasts transiently expressing the indicated BAK1 mutants,. b, d, f, Western blot analysis with α-BAK1 antibodies. For blot source data, see Supplementary Figure 1. c, Total ROS production following treatment of n=8 biologically independent leaf discs with 100 nM flg22 over 40 min. Circles indicate individual data points. a,c,e, Measurements are plotted as boxplots displaying the first and third quartiles, split by the median; whiskers extend to a maximum of 1.5 × interquartile range beyond the box. Outliers are indicated as black dots. Statistical analysis was performed using one-way ANOVA and Dunnett’s post-hoc test compared to WT BAK1. a-e, Experiments were repeated at least three times.