Figure 4 |. Expression of the BAK1-AAA and BAK1-S612A variants restores the semi-dwarf rosette phenotype of bak1–4 and bak1–4 bri1–301.

a, b, Representative images of rosettes from 4- to 5-week-old plants. c, d, Left, representative images of rosettes from 5- to 7-week-old plants. Right, western blot analysis with α-BAK1 antibodies. Blots were stained with Coomassie brilliant blue (CBB) for loading control. Experiments were repeated at least twice with similar results. Control plants used in (b), were previously pictured ³⁰. e, BES1 de-phosphorylation 60 min after treatment of 10-day-old seedlings with the indicated concentrations of epi-BL, as shown by western blot analysis using α-BES1 antibodies. Blots were probed with α-BAK1 antibodies, and subsequently stained with Coomassie brilliant blue (CBB) as loading controls. a-e, Experiments were repeated at least three times. For blot source data, see Supplementary Figure 1.