Table 1.
Primer/probe set | Nucleotide sequences | PCR product length, b.p. |
---|---|---|
1 | Forward primer GAATTGCAGCCATCGTGAATC TaqMan probe FAM-TCGTTTCCATCTTGCGCGTGC-BHQ1 Reverse primer CTCCGACGCTCTTTACACG |
149 |
2a | Forward primer GCGAAATGCGATACCTAGTG TaqMan probe FAM-TGCAGCCATCGTGAATCATCGAGT-BHQ1 Reverse primer GATGGAAACGACGCTCAAA |
114 |
3 | Forward primer GCGGACGACGTGTAAAG TaqMan Probe FAM-GAGCGAGTGTTGCGAGACAACAA-BHQ1 Reverse primer CGGGTATTCCTACCTGATTTG |
156 |
4 | Forward primer GAGCGTCGTTTCCATCTT TaqMan probe FAM-CAGCTCCGACGCTCTTTACACGTC-BHQ1 Reverse primer CAGCTTCGCTCCCTTTC |
102 |
5 | Forward primer GATCTCTTGGTTCTCGCATC TaqMan probe FAM-CACACTAGGTATCGCATTTCGCTGC-BHQ1 Reverse primer GCGTTCAAGAACTCGATGA |
92 |
6 | Forward primer GCAGCCATCGTGAATCAT TaqMan probe FAM-TTTGAGCGTCGTTTCCATCTTGCG-BHQ1 Reverse primer AGCTCCGACGCTCTTTA |
146 |
a The most optimal primer/probe sets for identification of P. kudriavzevii