. 2018 Nov 22;8:17252. doi: 10.1038/s41598-018-35092-1

Table 1.

Average number of biomass components whose production is affected by a full gene knockout.

		E. coli ^c	S. cerevisiae ^c
Number of biomass components		50	35
Standard model	Pleiotropy^a	10.0 (3)	12.1 (5)
Standard model	Essentiality^a	4.6 (2)	9.3 (2)
Free NADPH^b	Pleiotropy^a	9.9 (3)	11.1 (4.5)
Free NADPH^b	Essentiality^a	4.3 (2)	8.0 (1)

^aIn the FBA calculations, fluxes are either constrained to not exceed the wildtype (WT) fluxes to estimate the de facto contribution of gene products to biomass production (Pleiotropy), or they are allowed to vary freely to assess the number of biomass components for which gene products are essential even after allowing the mutant strain to adapt (Essentiality).

^bSolution when allowing unlimited conversion of NADPH to NADP+ ^cMean (median) number of affected biomass components.