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. 2018 Nov 22;8:17274. doi: 10.1038/s41598-018-35403-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of a panel of HIV-1 subtypes. Heatmap of HIV-1 DNA assays analyzed on DNA of a panel of HIV-1 patient isolates of different HIV-1 subtypes by ddPCR. Relative HIV-1 DNA quantification results per sample show the assays with high sensitivity (red) and the assays with lower sensitivity (blue). (*) DNA samples restricted with restriction enzyme XhoI instead of EcoRI prior ddPCR quantification.