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. 2018 Nov 22;9:4934. doi: 10.1038/s41467-018-07119-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Hormonal stimulation experimental design and effects on the trans-epithelial electrical resistance (TEER) and paracellular permeability (Papp). a Experimental design for mimicking the luteal and pre-ovulatory phases. b TEER measurements in the microfluidic devices under the three conditions; values were adjusted for the resistance found in an empty device (F = 14.503, p < 000.1; ANOVA followed by a post-hoc Tukey test). c Apparent permeability (Papp) of 0.4 and 4.4 kDa fluorescent markers in devices without cells (no cell) or in the presence of bovine oviductal epithelial cells (BOECs) under the three experimental conditions (F = 0.537, p = 0.590 and F = 0.583, p = 0.564 for 0.4 and 4.4 kDa, respectively; ANOVA followed by a post-hoc Tukey test). Graphs of b and c display average + s.d. Different letters (a vs b in b; a vs b and A vs B in c) indicate statistically significant differences (n = 6 devices per condition and pool, p < 0.05)