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. 2018 Nov 22;9:4934. doi: 10.1038/s41467-018-07119-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Effects of hormone stimulation of 3D-cultured BOECs on cell height, ciliation and oviductal glycoprotein 1 (OVGP1) expression under control, and simulated luteal and pre-ovulatory conditions. a Top and middle figures: 3D reconstruction of confocal immunofluorescent (IF) images for cilia (acetylated alpha-tubulin, green), nuclei (HOECHST 33342, blue), and actin filaments (phalloidin, red); bottom figures: IF for nuclei (blue) and OVGP1 (yellow). b Quantification of cell height in the different groups (F = 697.51, p < 0.0001; ANOVA followed by a post-hoc Tukey test). c Average percentage of ciliated cells for each group (F = 20.415, p = 0.002; ANOVA followed by a post-hoc Tukey test). d Quantification of OVGP1 expression adjusted for cell number (F = 12.52, p < 0.0001; ANOVA followed by a post-hoc Tukey test). Graph of b displays average ± s.d and graphs of c and d display average + s.d. Different letters (a vs b vs c in b; a vs b in c and d) indicate statistically significant differences (n = 6 devices per condition and pool, p < 0.05). Scale bars represent 10 µm