Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 22;8:17246. doi: 10.1038/s41598-018-35589-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

MS/MS quality and genomic coverage. (a) Circos graph representation of B. subtilis genome, including the annotated and potentially novel ORFs expressed in this study. (b) Venn diagram illustrating the MS coverage at peptide and protein levels in context of B. subtilis genome. (c) The genomic region visualization for seq_51322. Top panel includes known ORFs (in blue for + strand and in red for − strand) and all ORFs generated from six-frame genome translation (in green for + strand and in orange for − strand); color lightness corresponds to whether ORF was identified or not in our data (dark color for expressed ORF and light color for non-expressed ORF). The middle panel is zoomed around the expressed novel ORF of interest for visualization of peptide sequences (in khaki are peptides mapping to novel ORF and in purple are peptides from known ORF) and RT-PCR nucleotide sequences at this genomic location. The bottom panel contains the MS/MS spectra of the top scoring novel PSM.