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. 2018 Nov 22;8:17262. doi: 10.1038/s41598-018-35579-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distinct transverse sections observed for ballistic validation. Cresyl violet staining has been used here to reveal nervous tissue structures. The DG is framed in red in the photomicrographs. Four and three transverse sections were selected for the validation of dorsal (A) and ventral (B) DG model of irradiation, respectively. Sections a, d, e and f are supposed to be in the non-irradiated field, whereas sections b, c and f are supposed to be in the irradiated zones when following targeted irradiation protocols.