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. 2018 Nov 22;8:17262. doi: 10.1038/s41598-018-35579-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ballistic validation for dorsal DG irradiation. Photomicrographs and quantifications were performed in brain section b (Fig. 3), which is supposed to be in the irradiated field following dorsal DG irradiation procedure. Panel (A) shows representative photographs of gamma-H2AX staining in DG cell nuclei of control mice and of mice 1Gy irradiated at dorsal DG and brain levels. Panel (B) shows enlargements of photographs represented in panel (A). Graph (C) represents the percentage of nuclei expressing at least one gamma-H2AX staining focus in controls, dorsal DG and brain irradiated animals, for each dose of IR. Data are represented as the mean ± sem of 3–4 animals per group and are normalized to their corresponding controls (*p < 0.05 vs controls).