Figure 2.

RGS2 expression is inhibited by hypoxia. (a) Overview of RGS2 expression in LNCaP descending mouse tumours representative of RGS2 expression in correlation to blood vessels, proliferation and hypoxia (Bar = 100 µm). (b) Representative western blot from cell lysates obtained from cells cultured in a hypoxic atmosphere (H) (1% O₂) for 72 hours and corresponding normoxic controls (N) (21% O₂). HIF1A controls for hypoxic response and β-actin included for protein loading. (c) Representative WB from cell lysates obtained after 72 hours of CoCl₂ treatment (T) and respective control (C). The blots were cropped. LNCaP and LNCaP-19 was run on the same gel for both experiments (Hypoxia and CoCl₂) and PC-3 and WPMY were run on the same gel. All gels were run under the same conditions. The original pictures of the full-length western blots can be found in Supplementary Fig. S3.