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. 2018 Nov 22;8:17256. doi: 10.1038/s41598-018-33742-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BvADH enzymes are targeted to the plastids in planta. Green fluorescence protein (GFP) was fused at the C-terminal end of BvADHα (left) and BvADHβ (middle) and transiently expressed in N. benthamiana leaves. Free GFP (right) was used as control for cytosolic localization. Representative images show GFP fluorescence and chlorophyll autofluorescence in green and purple, respectively. Merge (bottom) is a composite with GFP fluorescence and chlorophyll autofluorescence. Scale bars = 50 μm.