. 2018 Nov 16;9:2602. doi: 10.3389/fimmu.2018.02602

Table 3.

Immunophenotyping of peripheral blood cell subsets in patients with AD undergoing AHSCT.

CELL SUBSET	PHENOTYPE
Minimum panel for immune reconstitution analyses
Total CD3, CD4, or CD8 T cells	CD3⁺CD4⁺CD8⁻; CD3⁺CD4⁻CD8⁺
Recent thymic emigrants	CD3⁺CD4⁺CD31⁺CD45RA⁺CD45RO⁻
Naive T cells^a, ^b	CD3⁺CD4⁺(CD8⁺)CD45RA⁺CD45RO⁻CCR7⁺CD62L⁺
Central memory T cells^a, ^b	CD3⁺CD4⁺(CD8⁺)CD45RA⁻CD45RO⁺CCR7⁺CD62L⁺
Effector memory T cells^a, ^b	CD3⁺CD4⁺(CD8⁺)CD45RA⁻CD45RO⁺CCR7⁻CD62L⁻
Effector T cells^a, ^b	CD3⁺CD4⁺(CD8⁺)CD45RA⁺CD45RO⁻CCR7⁻CD62L⁻
Exausted T cells^c	CD3⁺CD4⁺CD8⁻PD-1⁺; CD3⁺CD4⁻CD8⁺PD-1⁺
Naive B cells	CD19⁺CD27⁻IgD⁺
Switched memory B cells	CD19⁺CD27⁺IgD⁻
Non-Switched memory B cells	CD19⁺CD27⁺IgD⁺
Plasma cells	CD19⁺CD27^highIgD⁻CD38^high
Regulatory B cells (Breg)	CD19⁺CD24^highCD38^high or CD19⁺CD24^highCD38^highIgM^highIgM^highCD5⁺CD10⁺CD20⁺CD27⁻CD1d^high
Regulatory T cells (nTreg)	CD4⁺CD25^highCD127⁻Foxp3⁺
Suppressor T cells (CD8)	CD8⁺CD28⁻CD57⁺PD1⁺
NK cells (cytotoxic)	CD3⁻ CD56^dimCD16⁺
NK cells (cytotoxic)	CD3⁻ CD56^brightCD16^+/−
iNKT cells	CD19⁻CD3⁺Vα24⁻Jα18 TCR⁺
Stem cell-like memory T cells (TSCM)	CD3⁻CD4⁺(CD8⁺)CD45RO⁻CD45RA⁺CCR7⁺CD27⁺CD28⁺CD95⁺CD122⁺
Other cell subsets for immune reconstitution analyses
Follicular T helper (Tfh)	CD3⁺CD4⁺CXCR5⁺PD-1⁺Bcl-6⁺FoxP3⁻ or CD3⁺CD4⁺CXCR5⁺PD-1⁺ICOS⁺
Th1 cells	CD3⁺CD4⁺CXCR3⁺CCR6⁻Tbet⁺ or CD3⁺CD4⁺CXCR5⁻CXCR3⁺
Th2 cells	CD3⁺CD4⁺CCR4⁺CCR6⁻GATA3⁺ or CD3⁺CD4⁺CXCR5⁻CCR4⁺CCR6⁻
Th17 cells	CD3⁺CD4⁺CCR6⁺CCR4⁺RORγt⁺ or CD3⁺CD4⁺CXCR5⁻CCR4⁺CCR6⁺CCR10⁻
Th22 cells	CD3⁺CD4⁺CCR10⁺CCR4⁺AHR⁺ or CD3⁺CD4⁺CXCR5⁻CCR4⁺CCR6⁺CCR10⁺
Monocytes (classic)	CD14⁺CD16⁻
Monocytes (inflammatory)	CD14⁺CD16⁺CD64^high CD32^low
Monocytes (patrolling)	CD14^lowCD16⁺
Myeloid-derived suppressor cells (MDSCs) granulocytic^d	Lin-CD14⁻HLADR⁻CD33⁺CD11b⁺
Myeloid-derived suppressor cells (MDSCs) monocytic^d	Lin-CD14⁺HLADR⁻CD11b⁺
Plasmocytoid dendritic cells^d	Lin⁻CD14⁻CD123⁺CD11c⁻
Conventional dendritic cells^d	Lin⁻CD14⁻CD123⁻CD11c⁺
Upon in vitro t or b cell activation
Signaling Pathways (Erk, p38MAPK)^e	CD3⁺CD4⁺CD8⁻ERK; CD3⁺CD4⁺CD8⁻p38MAPK
Cytokine-producing CD4⁺ ou CD8⁺ T cells	CD3⁺CD4⁺(CD8⁺) IL2^−/+IL4^−/+IL17^−/+TNFα^−/+IL10^−/+IFNγ^−/+
IL-10-producing Breg cells	CD19⁺CD24^highCD38^highIL10⁺
IL-10-producing Treg cells	CD4⁺CD25^highCD127⁻Foxp3⁺IL10⁺

CD4⁺(CD8⁺) = CD4⁺ or CD8⁺ T cells.

CD45RO and CD62L are not essential to the panel.

Other exhaustion markers (such as TIM-3, LAG-3) or activation markers (CD69, HLA-DR) can be used.

Lineage (Lin) cocktail: CD3/CD19/CD20/CD56. Immunophenotyping panels were based on previous reports (12, 66, 67, 68, 69).