Figure 1. Comparison of flux profiling approaches.

The modeling approach can be classified into local, which estimates fluxes from proximal labeling patterns, and global, which estimates fluxes in a large-scale network. Experimental setup can be grouped by metabolic steady state and isotopic stationarity. Specific requirements or drawbacks of the different approaches are stated in the corresponding box. Key advantages (↑) and drawbacks (↓) of the different approaches are marked. References to computational methods for flux estimation are shown in white boxes.