Table 1.
Sequence of primers used for detection of virulence genes.
| Target gene | Product size (bp) | PCR primers sequence (5′-3′) | References |
|---|---|---|---|
| empA | 248 | F: CCTTTAACCAAGTGGGCGTA | Chen et al., 2005 |
| R: CGATTTGTAAGGGCGACAAT | |||
| vah1 | 493 | F: TGCGCTATATTGTCGATTTCAGTT | Rodkhum et al., 2006 |
| R: GCACCCGTTGTATCATCATCTAAG | |||
| vah2 | 876 | F: ATGAACGAAGATAACCCCCAGA | GenBank AB189395 |
| R: TCACTCTTCTGCTATCACTGG | |||
| vah3 | 1128 | F: ATGACTTCTTCTAAATTTTCGTTATGTGCG | GenBank AB189396 |
| R: GATAGAGCGGACTTTGCTTG | |||
| vah4 | 603 | F: ATGAAAACCATACGCTCAGCATCT | Rodkhum et al., 2006 |
| R: TCACGCTTGTTTTTGGTTTAAATGAAATCG | |||
| vah5 | 1758 | F: ATGCTCACGATAAGCCCTTTTAGAT | GenBank AB189398 |
| R: TCAAGGGTTAGGCGCGTGAT | |||
| rtxA | 441 | F: GCCTTCTTCGCCTAAACCT | GenBank EU155486 |
| R: ATTCGCAGCCACTACCAG | |||
| flaA | 435 | F: GTGCTGATGACTTCCGTATGG | Lu, 2010 |
| R: GCTCTGCCCGTTGTGAATC | |||
| flaD | 425 | F: TGACAGCACAGCGTTACCT | McGee et al., 1996 |
| R: GTTATCCGCACCGATTTG | |||
| flaE | 431 | F: CAGCCTGCTTCAGCGTAT | McGee et al., 1996 |
| R: TTTGCCCATTGATGTAGGT | |||
| virC | 344 | F: TCCTTCCTTGTGGTTAGCATTG | Lu, 2010 |
| R: GCCTCCGCAATAATCCAGTC | |||
| tonB | 195 | F: GGCGTAGAAGGTTTCGTT | GenBank AY644719 |
| R: CTCCACAGTCACGGTTTG | |||
| mreB | 711 | F: GCGTGATTGCGGATTTC | GenBank DQ907406 |
| R: CGACTGGTATTCCCGTTTC | |||
| toxR | 397 | F: AACACCACCAACGAGCCT | GenBank AB042547 |
| R: GACCACCAGTCGCAATCA | |||
| rpoS | 492 | F: CAAAGCGATGACGATG | GenBank AY695434 |
| R: TTCTTCTGCGGTAGGTTC | |||
| ftsZ | 185 | F: ATTTGCGAGTGCGAATGA | GenBank DQ907334 |
| R: CCATCTCTGCCGCTTCT | |||
| angM | 453 | F: TGAAGTTGAGCCTCGTAA | Lu, 2010 |
| R: TCAGACCTGTTGATTCGT | |||
| angR | 957 | F: AAGAGTGAGCCAATGCGTAG | Chen et al., 1996 |
| R: CTCCGAATCCATAACGATGA | |||
| virA | 314 | F: TCAGAGAGGATTGATAGGT | Lu, 2010 |
| R: ACACTTATGGGATGTAACAC | |||
| virB | 496 | F: GTAGAACGGCAGATTGGT | Lu, 2010 |
| R: GAGCCATAGCGATAGATTG |