Figure 2.

Differentiation status of lung T_RM and TILs. (A–D) The expression of CD45RA, CD27, and CD28 on CD4⁺ and CD8⁺ lung T_RM and TILs was determined. (A,B) The expression of CD27, CD45RA, and CD28 tumor CD103⁺ (top panels), CD69⁺ (middle panels), and CD69⁻ (bottom panels) CD4⁺ (A) and CD8⁺ (B) T cells shown by representative contour plots (CD45RA on y-axis, CD27 on x-axis) and histograms overlays (maximum set to 100%) show the expression of CD28 on the different subsets (black CD27⁻CD45RA⁻, gray CD27⁺CD45RA⁻, blue CD27⁻CD45RA⁺, purple CD27⁺CD45RA⁺). (C,D) The frequencies of CD27⁺CD45RA⁺CD28⁺ (light purple), CD27⁺CD45RA⁺CD28⁻ (medium purple), CD27⁺CD45RA⁻CD28⁺ (dark purple), CD27⁺CD45RA⁻CD28⁻ (light gray), CD27⁻CD45RA⁻CD28⁺ (medium gray), CD27⁻CD45RA⁻CD28⁻ (black), CD27⁻CD45RA⁺CD28⁺ (light blue), CD27⁻CD45RA⁺CD28⁻ (dark blue) of CD103⁺, CD69⁺, and CD69⁻ lung CD4⁺ (C; left bar graph), tumor CD4⁺ (C; right bar graph), lung CD8⁺ (D; left bar graph), and tumor CD8⁺ (D; right bar graph). (C,D) The quantifications are shown as bar graphs with the mean. n = 15.