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. 2018 Nov 16;9:2654. doi: 10.3389/fimmu.2018.02654

Figure 4.

Figure 4

Expression of T-bet, Eomes and GZMB on TRM and TILs. (A–D) The expression of T-bet, Eomes, and GZMB was analyzed on CD4+ and CD8+ TRM and TILs. The expression of T-bet (top panels), Eomes (middle panels), and GZMB (bottom panels) on lung (left panels) and tumor (right panels) CD4+ (A) and CD8+ (B) T cells is shown by representative histogram overlays (maximum set to 100%) (CD103+ black, CD69+ dark gray, CD69− solid light gray). The expression of T-bet (geometric mean fluorescence intensity; GeoMFI) (C) and frequencies of Eomes+ (D) and GZMB+ (E) CD103+ (black circles), CD69+ (dark gray circles), and CD69− (light gray circles) cells of lung and tumor CD4+ (left graphs) and CD8+ (right graphs) T cells. (C–E) The quantifications are shown as dot plots with the horizontal line indicating the mean and each point represents a unique sample. n = 17. Open circles and solid circles indicate adeno- and squamous carcinoma, respectively. **p < 0.01, ****p < 0.0001; 2-way ANOVA with Tukey's multiple comparisons test.