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. 2018 Nov 16;9:2654. doi: 10.3389/fimmu.2018.02654

Figure 5.

Figure 5

CD103+ TILs express the highest levels of inhibitory receptors. (A–I) The expression of inhibitory receptors PD-1, CTLA-4, and 2B4 was analyzed on CD4+ and CD8+ TRM and TILs. The expression of PD-1 (A), CTLA-4 (D), and 2B4 (G) on lung (left panel) and tumor (right panel) on CD4+ (top panel) and CD8+ (bottom panel) T cells is shown by representative histogram overlays (maximum set to 100%) (CD103+ black, CD69+ dark gray, CD69− solid light gray). The frequencies and geoMFI of PD-1+ (B,C), CTLA-4+ (E,F) and 2B4+ (H,I) were quantified for CD103+ (black circles), CD69+ (dark gray circles), and CD69− (light gray circles) cells of lung and tumor CD4+ (left graphs) and CD8+ (right graphs) T cells. (B,C,E,F,H,I) The quantifications are shown as dot plots with the horizontal line indicating the mean and each point represents a unique sample. n = 17. Open circles and solid circles indicate adeno- and squamous carcinoma, respectively. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; 2-way ANOVA with Tukey's multiple comparisons test.