Figure 7.

Co-stimulation enhances cytokine production of TILs. (A–F) The effect of co-stimulation on cytokine production of TILs was assessed with overnight stimulation. (A–C) The frequencies of IFN-γ⁺ (A), TNFα⁺ (B), or TNF-α⁺IFN-γ⁺ (C) CD103⁺, CD69⁺, and CD69⁻ CD4⁺ TILs stimulated with αCD3 with control cell line (CL) (black circles), αCD3 and αCD28 with control CL (black squares), or αCD3 and αCD28 with CD70-expressing CL (black triangles) was quantified. (D–F) The frequencies of IFN-γ⁺ (D), TNFα⁺ (E), or TNF-α⁺IFN-γ⁺ (F) CD103⁺, CD69⁺, and CD69⁻ CD8⁺ TILs stimulated with αCD3 with control CL (black circles), αCD3 and αCD28 with control CL (black squares), or αCD3 and αCD28 with CD70-expressing CL (black triangles) was quantified. n = 5. ^*p < 0.05, ^**p < 0.01, ^****p < 0.0001; 2-way ANOVA with Tukey's multiple comparisons test.