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. 2018 Nov 16;8:407. doi: 10.3389/fcimb.2018.00407

Figure 2.

Figure 2

Number of unique and shared identifications for proteins detected as significantly upregulated in the virulent (A) and attenuated (B) cultivated H. meleagridis strain by the 2D-DIGE-α, 2D-DIGE-β, and SWATH MS experiments. Venny 2.1.0 tool (Oliveros, 2015) was used to summarize the findings with venn diagrams. The same extracted protein samples were analyzed by the 2D-DIGE-α and SWATH MS experiment. Based on the analyses the following identified proteins were found to be common between experiments: ♦ = actin, GAPDH and PEPCK (A); ▴ = Clan CD, family C13, asparaginyl endopeptidase-like CP (A); = GAPDH (B); ■ = Coronin (B). 2D-DIGE, two-dimensional differential gel electrophoresis; CP, cysteine peptidase; SWATH MS, sequential window acquisition of all theoretical mass spectra mass spectrometry; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PEPCK, phosphoenolpyruvate carboxykinase.