Table 1.
Experimental design for the 2D-DIGE-α and 2D-DIGE-β experiments.
| Gel Nr. | Fluorescent dyes | ||
|---|---|---|---|
| G-Dye100 | G-Dye200 | G-Dye300 | |
| 1 | IS (50 μg) | 25a (50 μg) | 303b (50 μg) |
| 2 | IS (50 μg) | 25b (50 μg) | 303a (50 μg) |
| 3 | IS (50 μg) | 303b (50 μg) | 25a (50 μg) |
| 4 | IS (50 μg) | 303a (50 μg) | 25b (50 μg) |
IS, Internal standard.
25a, Biological replicate 1 of virulent Histomonas meleagridis parasites, of passage number 25, protein extraction on day 1.
25b, Biological replicate 2 of virulent H. meleagridis parasites, of passage number 25, protein extraction on day 2.
303a, Biological replicate 1 of attenuated H. meleagridis parasites, of passage number 303, protein extraction on day 1.
303b, Biological replicate 2 of attenuated H. meleagridis parasites, of passage number 303, protein extraction on day 2.