Table 1.
Bacterial strains and plasmids used in this study.
| Strains | Relevant description | Source/Reference |
|---|---|---|
| C. jejuni 81–176 WT | Wild type strain of C. jejuni | Dr. Qijing Zhang |
| Δtlp2 | C. jejuni 81–176 derivative with deletion in tlp2 gene; tlp2::kan | This study |
| Δtlp2-cm | C. jejuni 81–176 derivative with deletion in tlp2 gene; tlp2::cm | This study |
| tlp2 comp | C. jejuni 81–176 tlp2 mutant complemented with wild type copy of tlp2 on pRY112 | This study |
| C. jejuni NCTC11168Δfur | C. jejuni NCTC11168 derivative with deletion in fur gene; fur::tet | Dr. Jun Lin |
| WT Ptlp2-pMW10 | C. jejuni 81–176 WT reporter strain carrying Ptlp2-pMW10 | This study |
| Δtlp2 Ptlp2-pMW10 | C. jejuni 81–176 Δtlp2 strain carrying Ptlp2-pMW10 | This study |
| Δfur Ptlp2-pMW10 | C. jejuni 81–176 Δfur strain carrying Ptlp2-pMW10 | This study |
| WT Pint-pMW10 | C. jejuni 81–176 WT reporter strain carrying Ptlp2-phoXint-pMW10 | This study |
| E. coli DH5α | E. coli strain used for cloning | Invitrogen |
| Plasmids | ||
| pZero-1 | Cloning vector for making suicide vector; Zeo | Invitrogen |
| pUC4K | Source plasmid for kanamycin resistance gene; Kan | Amersham |
| pUC4C | Source plasmid for chloramphenicol resistance gene; Cm | This study |
| pMW10 | Promoter shuttle vector; pMW10, Kan | Wosten et al., 1998 |
| pRY112 | E.coli-Campylobacter shuttle vector for complementation; Cm | Yao et al., 1993 |
| pRK2013 | Helper plasmid for complementation; Kan | Grabowska et al., 2011 |
| pZero1-tlp2 | pZero-1 containing the upstream and downstream sequences of tlp2; Zeo | This study |
| pZero1-Δtlp2-kan | pZero1-tlp2 with tlp2 gene replaced by the pUC4K kan gene through inverse PCR; Zeo, Kan | This study |
| pZero1-Δtlp2-cm | pZero1-tlp2-kan where kanwas replaced by the chloramphenicol gene; Zeo, Cm | This study |
| Ptlp2-pMW10 | pMW10 carrying the tlp2 promoter; Kan | This study |
| Pint-pMW10 | pMW10 carrying the intergenic region between tlp2 and phoX; Kan | This study |
Cm, chloramphenicol resistance; Kan, kanamycin resistance; Zeo, Zeocin resistance.