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. 2018 Nov 16;9:2759. doi: 10.3389/fmicb.2018.02759

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Copyright © 2018 Pedersen, Bombar, Paerl and Riemann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative abundance of the main nifH gene based operational taxonomic units (OTUs) each accounting for > 1% of reads in any sample in the colonization experiment. Only selected OTU labels are shown. NifH gene clusters are marked by horizontal lines (Cluster I) and checkered pattern (Cluster III), and vertical lines (Cluster IV). NifH subclusters according to Zehr et al. (2003) are indicated and the phylogenetic assignments are: 1G and 1O (unassigned); 1K (Beta-Proteobacteria); Subclusters of nifH Cluster III are putative anaerobic bacteria. BLAST hits for predominant OTUs are shown in Supplementary Table S3. In some cases, less than the maximum number of replicates per sample is shown since amplification was unsuccessful for single replicates.