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. 2018 Nov 16;9:1617. doi: 10.3389/fphys.2018.01617

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Copyright © 2018 Li, Zhang, Zhu, Qi, Lin and Cheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescence staining of TUNEL/NeuN/DAPI. (A) Immunofluorescence staining of TUNEL positive neurons. The white arrow indicted the apoptotic neurons (Scale bars = 25 μm). (B) The ratio of apoptotic neurons increased remarkably after SCI. However, high dose of ICA dramatically decreased the ratio of TUNEL positive neurons. N = 6. ^∗P< 0.01 compared to SCI group.