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. 2018 Nov 23;108(5):1135–1144. doi: 10.1093/ajcn/nqy166

TABLE 4.

Regression of n–6 levels (wt% of cord plasma glycerophospholipids) on each of 14 SNPs of the FADS1–3 regions1

Fatty acid
C18:2n–6 ln(C18:3n–6) C20:2n–6 C20:3n–6 C20:4n–6 ln(C22:4n–6) C22:5n–6 C20:3n–6/C18:2n–6 ln(C20:4n–6/C20:3n–6) ln(C20:4n–6/C18:2n–6)
FADS1 rs174548 β coefficient −0.562 0.062 −0.046 −0.835 1.186 0.055 0.080 −0.053 0.233 0.144
P value 6.97 × 10–10*** 5.28 × 10-04 1.89 × 10-06*** 4.51 × 10-42*** 3.71 × 10-21*** 8.72 × 10-05*** 2.42 ×10-03 6.44 × 10-08*** 1.58 × 10-45*** 3.97 × 10-22***
R 2, % 9.2 2.8 5.5 37.9 20.4 3.7 2.1 7.1 40.4 21.3
Intergenic rs3834458 β coefficient −0.659 0.059 −0.053 −0.877 1.298 0.060 0.101 −0.050 0.249 0.163
P value 7.39 × 10-12*** 1.66 ×10-03 2.1 × 10-07*** 4.72 × 10-41*** 1.33 × 10-22*** 5.38 × 10-05*** 3.21 × 10-04 1.71 × 10-06*** 2.49 × 10-46*** 1.97 × 10-25***
R 2, % 11.2 2.3 6.5 37.2 21.8 3.9 3.1 5.5 41.0 24.4
FADS2 rs174570 β coefficient −0.650 0.059 −0.053 −0.795 1.249 0.051 0.084 −0.042 0.230 0.158
P value 5.8 × 10-12*** 1.35 ×10-03 1.06 × 10-07*** 6.23 × 10-34*** 1.2 × 10-21*** 5.05 × 10-04 2.37 × 10-03 6.06 × 10-05*** 5.29 × 10-40*** 5.7 × 10-25***
R 2, % 11.3 2.4 6.8 31.6 20.9 2.8 2.1 3.8 36.4 23.9
rs174574 β coefficient −0.674 0.058 −0.052 −0.870 1.297 0.056 0.099 −0.048 0.247 0.165
P value 3.44 × 10-12*** 2.27 ×10-03 3.9 × 10-07*** 2.06 × 10-39*** 3.45 × 10-22*** 1.74 × 10-04*** 4.79 × 10-04 5.39 × 10-06*** 1.26 × 10-44* 1.33 × 10-25***
R 2, % 11.6 2.1 6.2 35.9 21.4 3.3 2.9 5.0 39.8 24.5
rs2727271 β coefficient 0.355 −0.022 0.021 0.403 −0.760 −0.016 −0.045 0.018 −0.124 −0.093
P value 1.34 × 10-05*** 1.6 ×10-01 1.38 × 10-02 9.54 × 10-12*** 2.79 × 10-11*** 1.96 × 10-01 5.37 × 10-02 4.52 × 10-02 3.79 × 10-15*** 5.9 × 10-12***
R 2, % 4.6 0.3 1.3 11.1 10.6 0.2 0.7 0.8 14.6 11.3
rs174576 β coefficient −0.669 0.056 −0.051 −0.851 1.251 0.056 0.101 −0.046 0.241 0.161
P value 3.48 × 10-12*** 2.86 ×10-03 6.32 ×10-07*** 4.3 × 10-38*** 7.09 × 10-21*** 1.83 × 10-04*** 3.15 × 10-04 1.11 × 10-05*** 1.47 × 10-42*** 7.55 × 10-25***
R 2, % 11.6 2.0 6.0 34.9 20.2 3.3 3.1 4.6 38.3 23.8
rs174579 β coefficient 0.130 −0.020 0.018 0.233 −0.140 −0.026 −0.028 0.018 −0.053 −0.023
P value 1.63 × 10-01 2.66 × 10-01 5.54 × 10-02 6.2 × 10-04 2.92 × 10-01 6.02 × 10-02 2.97 × 10-01 6.54× 10-02 3.82× 10-03 1.42 × 10-01
R 2, % 0.2% 0.1% 0.7% 2.7% 0.0% 0.7% 0.0% 0.6% 1.9% 0.3%
rs174602 β coefficient −0.220 0.021 −0.016 −0.375 0.527 0.027 0.047 −0.028 0.105 0.059
P value 7.99 × 10-03 1.85× 10-01 6.04 × 10-02 3.71 × 10-10*** 6.56 × 10-06*** 2.96 × 10-02 4.73 × 10-02 1.64 × 10-03 8.12 × 10-11*** 2.26 × 10-05***
R 2, % 1.6% 0.2% 0.7% 9.4% 4.9% 1.0% 0.8% 2.3% 10.1% 4.3%
rs498793 β coefficient 0.111 −0.004 0.028 0.174 −0.311 −0.012 −0.029 0.013 −0.052 −0.031
P value 3.6× 10-01 8.67 × 10-01 2.35 × 10-02 4.99 × 10-02 6.96 X 10-02 5.14 × 10-01 3.97 × 10-01 3.07 × 10-01 3.15 × 10-02 1.27 × 10-01
R 2, % 0.0 −0.3 1.1 0.7 0.6 −0.1 −0.1 0.0 0.9 0.3
rs526126 β coefficient 0.056 −0.001 0.008 0.135 −0.424 0.009 0.022 0.012 −0.053 −0.033
P value 5.55 × 10-01 9.67 × 10-01 4. × 10-01 5.53 × 10-02 1.68 × 10-03 5.23 × 10-01 4.21 × 10-01 2.45 × 10-01 5.02 × 10-03 3.8 × 10-02
R 2, % −0.2 −0.3 −0.1 0.7 2.3 −0.2 −0.1 0.1 1.8 0.9
rs174575 β coefficient 0.138 −0.019 0.015 0.264 −0.136 −0.031 −0.040 0.021 −0.059 −0.024
P value 1.3× 10-01 2.7 × 10-01 1.21 × 10-01 7.5 × 10-05*** 2.96 x 10-01 2.42 × 10-02 1.26 x 10-01 2.98 × 10-02 1.21 × 10-03 1.23 × 10-01
R 2, % 0.3 0.1 0.4 3.7 0.0 1.1 0.3 1.0 2.4 0.4
Intergenic rs174448 β coefficient 0.129 −0.009 0.009 0.133 −0.103 −0.023 −0.065 0.004 −0.032 −0.023
P value 1.18 × 10-01 5.79 × 10-01 2.95 × 10-01 2.94 × 10-02 3.8 × 10-01 6.93 × 10-02 5.73 × 10-03 6.44 × 10-01 5.02 × 10-02 1. × 10-01
R 2, % 0.4 −0.2 0.0 1.0 −0.1 0.6 1.7 −0.2 0.7 0.4
Intergenic rs174449 β coefficient −0.158 0.015 −0.015 −0.206 0.183 0.021 0.065 −0.009 0.051 0.032
P value 4.99 × 10-02 3.44 × 10-01 7.25 × 10-02 4.85 × 10-04 1.11 × 10-01 8.95 × 10-02 4.72 × 10-03 2.85 × 10-01 1.5 × 10-03 1.74 × 10-02
R 2, % 0.7 0.0 0.6 2.9 0.4 0.5 1.8 0.0 2.3 1.2
FADS3 rs174455 β coefficient −0.173 0.007 −0.007 −0.162 0.130 0.018 0.066 −0.004 0.039 0.030
P value 3.5 × 10-02 6.45 × 10-01 4.29 × 10-01 7.36 × 10-03 2.68 × 10-01 1.51 × 10-01 4.5 × 10-03 6.66 × 10-01 1.75 × 10-02 2.97 × 10-02
R 2, % 0.9 −0.2 −0.1 1.6 0.1 0.3 1.8 −0.2 1.2 1.0

1Outcome is respective fatty acid wt% of cord plasma glycerophospholipids; ln indicates whether the outcome was log transformed to account for severe right skewness of the fatty acid distribution, or entered into the regression equation on the original scale. The β coefficient is the estimated regression coefficient from unadjusted linear regression of each fatty acid on each genetic variant of the FADS gene regions. The P values are uncorrected for multiple testing, with *** indicating significance after Bonferroni correction for multiple testing (critical P value is 1.84 × 10–4).