TABLE 5.
Regression of n–3 levels (wt% of cord plasma glycerophospholipids) on each of 14 SNPs of FADS1–3 regions1
| Fatty acid | ||||||||
|---|---|---|---|---|---|---|---|---|
| ln(C18:3n–3) | ln(C20:3n–3) | ln(C20:5n–3) | ln(C22:5n–3) | C22:6n–3 | ln(C20:5n–3/C18:3n–3) | |||
| FADS1 | rs174548 | β coefficient | −0.076 | 0.015 | 0.012 | 0.013 | 0.0260 | 0.088 |
| P value | 1.22 × 10–2 | 2.21 × 10−01 | 7.22 × 10−01 | 6.75 × 10−01 | 4.34 × 10−03 | 3.31 × 10−02 | ||
| R 2, % | 1.4 | 0.1 | −0.2 | −0.2 | 1.8 | 0.9 | ||
| Intergenic | rs3834458 | β coefficient | −0.096 | 0.014 | −0.012 | −0.003 | 0.318 | 0.083 |
| P value | 2.83 × 10−03 | 3.11 × 10−01 | 7.31 × 10−01 | 9.36 × 10−01 | 9.65 × 10−04 | 5.79 × 10−02 | ||
| R 2, % | 2.0 | 0.0 | −0.2 | −0.3 | 2.5 | 0.7 | ||
| FADS2 | rs174570 | β coefficient | −0.091 | 0.012 | −0.001 | −0.004 | 0.302 | 0.090 |
| P value | 3.95 × 10−03 | 3.43 × 10−01 | 9.8 × 10−01 | 8.93 × 10−01 | 1.45 × 10−03 | 3.69 × 10−02 | ||
| R 2, % | 1.9 | 0.0 | −0.3 | −0.3 | 2.3 | 0.9 | ||
| rs174574 | β coefficient | −0.100 | 0.012 | −0.007 | −0.010 | 0.299 | 0.093 | |
| P value | 1.89 × 10−03 | 3.89 × 10−01 | 8.38 × 10−01 | 7.63 × 10−01 | 2.15 × 10−03 | 3.55 × 10−02 | ||
| R 2, % | 2.2 | −0.1% | −0.2 | −0.2 | 2.2 | 0.9 | ||
| rs2727271 | β coefficient | 0.070 | −0.016 | 0.000 | 0.005 | −0.178 | −0.071 | |
| P value | 8.34 × 10−03 | 1.55 × 10−01 | 9.97 × 10−01 | 8.43 × 10−01 | 2.73 × 10−02 | 5.32 × 10−02 | ||
| R 2, % | 1.5 | 0.3 | −0.3 | −0.2 | 1.0 | 0.7 | ||
| rs174576 | β coefficient | −0.101 | 0.010 | −0.009 | −0.008 | 0.296 | 0.093 | |
| P value | 1.53 × 10−03 | 4.43 × 10−01 | 8.14 × 10−01 | 7.98 × 10−01 | 2.23 × 10−03 | 3.4 × 10−02 | ||
| R 2, % | 2.3 | −0.1 | −0.2 | −0.2 | 2.1 | 0.9 | ||
| rs174579 | β coefficient | −0.001 | 0.11 | 0.004 | 0.001 | −0.033 | 0.005 | |
| P value | 9.76 × 10−01 | 3.66 × 10−01 | 9.09 × 10−01 | 9.86 × 10−01 | 7.2 × 10−01 | 9.08 × 10−01 | ||
| R 2, % | −0.3 | 0.0 | −0.3 | −0.3 | −0.2 | −0.3 | ||
| rs174602 | β coefficient | −0.046 | −0.012 | −0.012 | −0.028 | 0.076 | 0.034 | |
| P value | 8.7 × 10−02 | 2.76 × 10−01 | 6.85 × 10−01 | 2.9 × 10−01 | 3.53 × 10−01 | 3.58 × 10−01 | ||
| R 2, % | 0.5 | 0.0 | −0.2 | 0.0 | 0.0 | 0.0 | ||
| rs498793 | β coefficient | 0.034 | 0.009 | 0.036 | 0.018 | 0.043 | 0.002 | |
| P value | 3.92 × 10−01 | 5.65 × 10−01 | 4.19 × 10−01 | 6.45 × 10−01 | 7.17 × 10−01 | 9.69 × 10−01 | ||
| R 2, % | −0.1 | −0.2 | −0.1 | −0.2 | −0.2 | −0.3 | ||
| rs526126 | β coefficient | 0.071 | 0.020 | −0.049 | −0.005 | −0.137 | −0.120 | |
| P value | 2.22 × 10−02 | 1.15 × 10−01 | 1.59 × 10−01 | 8.84 × 10−01 | 1.43 × 10−01 | 4.41 × 10−03 | ||
| R 2, % | 1.1 | 0.4 | 0.3 | −0.3 | 0.3 | 1.8 | ||
| rs174575 | β coefficient | −0.001 | 0.010 | 0.015 | 0.008 | −0.016 | 0.015 | |
| P value | 9.85 × 10−01 | 4.25 × 10−01 | 6.55 × 10−01 | 7.87 × 10−01 | 8.61 × 10−01 | 7.02 × 10−01 | ||
| R 2, % | −0.3 | −0.1 | −0.2 | −0.2 | −0.3 | −0.2 | ||
| Intergenic | rs174448 | β coefficient | 0.129 | −0.009 | 0.009 | 0.133 | −0.103 | −0.023 |
| P value | 1.18 × 10−01 | 5.79 × 10−01 | 2.95 × 10−01 | 2.94 × 10−02 | 3.8 × 10−01 | 6.93 × 10−02 | ||
| R 2, % | 0.4 | −0.2 | 0.0 | 1.0 | −0.1 | 0.6 | ||
| Intergenic | rs174449 | β coefficient | −0.020 | 0.007 | −0.044 | −0.028 | 0.006 | −0.023 |
| P value | 4.38 × 10−01 | 5.02 × 10−01 | 1.37 × 10−01 | 2.91 × 10−01 | 9.38 × 10−01 | 5.12 × 10−01 | ||
| R 2, % | −0.1 | −0.1 | 0.3 | 0.0 | −0.3 | −0.1 | ||
| FADS3 | rs174455 | β coefficient | −0.024 | −0.002 | −0.053 | −0.036 | −0.007 | −0.029 |
| P value | 3.77 × 10−01 | 8.34 × 10−01 | 7.75 × 10−02 | 1.71 × 10−01 | 9.34 × 10−01 | 4.2 × 10−01 | ||
| R 2, % | −0.1 | −0.2 | 0.5 | 0.2 | −0.3 | −0.1 | ||
1Outcome is respective fatty acid (wt% of cord plasma glycerophospholipids); ln indicates whether the outcome was log transformed to account for severe right skewness of the fatty acid distribution, or entered into the regression equation on the original scale. β Coefficient is the estimated regression coefficient from unadjusted linear regression of each fatty acid on each genetic variant of the FADS gene regions. The P values are uncorrected for multiple testing (critical P value is 1.84 × 10–4). FADS, fatty acid desaturase; SNP, single-nucleotide polymorphism.