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The effect of ibrutinib on NF-κB pathway during 3 doses of ibrutinib. Immunoblot analyses of (A) NF-κB pathway proteins in CLL cells from patients 2, 3, 7, and 8 on D28 of each cycle. (B) Mean levels (n = 4) of the proteins shown in (A). Protein levels were normalized to both β-actin and GAPDH, averaged and then expressed relative to pretreatment levels. Error bars represent SEMs.
