Subject area	Environmental Science
More specific subject area	Soil microbial ecology
Method name	High-throughput PLFA analysis
Name and reference of original method	J.S. Buyer, M. Sasser, High-throughput phospholipid fatty acid analysis of soils, Appl. Soil Ecol. 61 (2012) 127-130.
Resource availability	-Freeze-drier and pump (e.g. Alpha 2-4 LD, Christ, Germany; Vacuubrand 2.5, Germany). -Internal standard (e.g. 19:0 phosphatidylcholine, Avanti Polar Lipids, Alabaster, AL, US). -External standard (e.g. Bacterial Acid Methyl Ester, Supelco, Sigma Aldrich, US). -Solid phase extraction columns (e.g. HyperSep™ SI columns, 50 mg/1 ml, Thermo Scientific, US). -Centrifugal evaporator with vacuum pump, cold trap and rotor (e.g. miVac Duo Concentrator; miVac Duo Pump; miVac Speed Trap; rotor DRC-13100-032, GeneVac, UK). Nitrogen evaporation could be used instead of this equipment, if required. -GCMS, column and processing software (e.g. Trace GC Ultra and DSQ II, Thermo Scientific, US; ZB-FFAP, Phenomenex®,Macclesfield, UK; Xcalibur®, Thermo Fisher Scientific, US).