Fig. 3.

pRAD50(Ser635) quantifies ATM pathway induction by irinotecan and ATM inhibition by AZ31 in an SW620 colorectal xenograft model. a Representative images of pRAD50(S635) staining in SW620 xenograft tissue, treated with irinotecan and fixed after 3 h (50 mg/kg intraperitoneal) or AZ31 (100 mg/kg oral) in combination or with a vehicle control. b HALO image analysis of the IHC stained samples was used to generate a pRAD50 H-score per sample. c Western blot analysis was used to semi-quantify the level of pATM(S1981) protein in each xenograft sample. Mean ± SD given. Each data point indicates an individual mouse. *** indicates p ≤ 0.001 by One-way ANOVA, followed by Tukey’s multiple comparison correction. Scale bars: 50 µm