Fig. 2.

miR-1247 inhibits anchorage dependent cell survival and proliferation by promoting cell apoptosis in hypermethylated colon cancer. a TaqMan RT-qPCR of miR-1247 expression level after 48 h of miR-1247 mimic transfection. Results are represented as a relative fold change normalised to the baseline miR1247 expression of respective cell line. b Anoikis assay: viability of the cells was visualised after 24 h of miR1247 or scrambled transfection followed by trypan blue staining in a 24-well plate precoated with 10% poly-HEMA. c Cell proliferation of methylator RKO cells transfected with miR-1247 mimic or mock miRNA was estimated by cell titer. d Cell proliferation of non-methylated SW480 cells transfected with miR-1247 mimic or mock miRNA. e, f Annexin V (early apoptosis marker) plus PI (late apoptosis marker and necrosis) staining of hypermethylated RKO cell line or non-methylated SW480 cell line transfected with miR-1247 mimic or mock miRNA 3 days after transfection