Fig. 3.

miR-1247 regulates colon cancer cell motility. a miR-1247 inhibits cancer cell migration in a transwell assay. The hypermethylated RKO cells were transfected with miR-1247 mimic or mock miRNA for 48 h before subjected to the transwell assay. The cells were seeded on a Matrigel precoated transwell membrane. The cells on the lower side of the chamber were stained, representing the number of cells that migrated. Cells treated with miR-1247 mimic exhibited less migration. Representative images are shown along with overall quantification. b Transwell assays of the non-methylated SW480 cells transfected with miR-1247 mimic or mock miRNA before subjected to the transwell assay and treatment and analysis are similar with transwell assay described above. c Transwell assays of the hypermethylated RKO cells transfected with anti-sense miR-1247 inhibitor or scrambled miRNA. After 48 h, cells (50,000) were subjected to transwell assay for 24 h. Cells transfected with the inhibitor demonstrated less migration. Representative images are shown along with overall quantification. d Transwell assays of the non-methylated SW480 cells was performed by transfecting cells with anti-sense miR-1247 inhibitor or scrambled miRNA. Histograms represent the quantification of the associated pictures in triplicates