Fig. 2.

Phylogenetic analysis of Glossina sp. field samples (▼) and reference Glossina species by comparison of partial COI sequences. 592 bp stretches of representative samples for each species and sampling spot were compared to sequences obtained from NCBI as described under Methods. Tamura-Nei-93-model assuming gamma distribution and invariant sites was used to construct the tree and was evaluated with 900 bootstrap replicates. Accession numbers of reference sequences are indicated after the species name. EG Equatorial Guinea, CAM Cameroon, CR Cross River National Park