Table 3.
Analysis of ITS1 sequence length, microsatellite markers and Wolbachia status in Glossina laboratory populations
| Glossina species | Country of origin (Location) | No | ITS1 expected size | Wolbachia | Microsatellites | Correctly identified samples | ||
|---|---|---|---|---|---|---|---|---|
| cytoplasmic | chromosomal | A10 | Gmm14 | |||||
| G. pallidipes | IPCL Uganda |
8 | 920 | 0.0 % (0/8) | 0.0 % (0/8) | - | + | 8/8 |
| IPCL Ethiopia |
8 | 12.5 % (1/8) | 0.0 % (0/8) | - | + | 8/8 | ||
| G. m. morsitans | IPCL | 8 | 775 | 75 % (6/8) | 100 % (8/8) | - | + | 8/8 |
| G. m. centralis | IPCL | 8 | ~800 + ~150 | 100 % (8/8) | 0.0 % (0/8) | - | + | 8/8 |
| G. m. submorsitans | CIRDES | 12 | ~800 + ~150 | 0.0 % (0/12) | 0.0 % (0/8) | - | + | 12/12 |
| G. p. gambiensis | IPCL POUT |
8 | 543 | 0.0 % (0/8) | 0.0 % (0/8) | + | + | 8/8 |
| IPCL CIRDES |
8 | 0.0 % (0/8) | 0.0 % (0/8) | + | + | 8/8 | ||
| G. f. fuscipes | IPCL Central Africa Republic |
8 | 618 | 12.5 % (1/8) | 0.0 % (0/8) | Partial | + | 8/8 |
| G. brevipalpis | IPCL | 8 | 778 | 75 % (6/8) | 0.0 % (0/8) | - | - | 8/8 |
| G. tachinoides | CIRDES | 12 | 597 | 0.0 % (0/12) | 0.0 % (0/8) | - | + | 12/12 |
-: no amplicon detected
+: the expected amplicon was detected in all individuals screened
Partial: the expected amplicon was detected, but not in all individuals screened