Table 4.

Validation of Tsetse species from field collected samples using Glossina ITS1

In grey scale: field collections lacking reference laboratory populations

^aBased on the ITS1 profile, non-amplification of microsatellite A10, complete absence of the cytoplasmic infection of Wolbachia, and the geographical distribution of tsetse species, these 7 samples were identified as G. m. submorsitans

^bBased on the ITS1 profile, amplification pattern of both A10 and Gmm14 microsatellite markers, absence of cytoplasmic and chromosomal Wolbachia, and the geographic distribution of tsetse species, these individuals were identified as G. tachinoides

^cBased on the ITS1 profile, amplification of both A10 and Gmm14 microsatellite, absence of cytoplasmic and chromosomal Wolbachia, and the geographic distribution of tsetse species, these individuals were identified as G. m. submorsitans

^dBased on the ITS1 profile, COI profile, amplification pattern of both A10 and Gmm14 microsatellite markers, absence of cytoplasmic and chromosomal Wolbachia, and the geographic distribution of tsetse species, these individuals were identified as G. pallidipes

^eFor G. swynnertoni, there was no ITS1 amplicon expected from previous studies. The one generated in the present study is stated as ‘expected’