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Impact of GpSGHV infection on G. f. fuscipes female insemination rate when mated with a: 3, 6, 9 or 12 day old untreated males; Proportion of empty spermathecae reduced significantly with male age (y = − 0.02165*x + 0.3207, P = 0.005); proportion partially filled (y = − 0.01257*x + 0.4139, P = 0.1577) and completely filled increased (y = 0.01040 * x + 0.25526, P = 0.04 between 3 and 12 days post emergence. b: 9-days old virgin males from different treatments
