Fig. 2.

Levels of miR-133b, EGFR, ITGB4, and FAK in ESCC cell lines. a The level of miR-133b, and mRNA levels of EGFR and ITGB4 in ESCC cell lines and normal human esophageal epithelial cell line were detected by RT-qPCR. b The protein levels of EGFR, ITGB4, p-FAK, and FAK in ESCC cell lines and normal human esophageal epithelial cell line were detected by western blotting. c The gray-scale value of the bands were quantitatively analyzed. d The specific binding sites of miR-133b in 3′-UTR of EGFR mRNA, and mutation binding sites were shown. WT, wild-type; MUT, mutant type. e Relative luciferase activities after co-transfection with the EGFR reporter and the miR-133b agomir or miR-133b NC for 48 h. The experimental data were representative of three independent experiments. Results were expressed as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001