Fig. 4.

MiR-133b repressed proliferation, anoikis resistance and anchorage-independent growth of ESCC cells via regulating EGFR. a Proliferation of KYSE150 and ECa109 cells in different treatment groups was assessed by MTT assay. b Anoikis of KYSE150 and ECa109 cells that were seeded into poly-Hema pre-coated plates was detected by Annexin V/PI staining. c The anoikis rate of KYSE150 and ECa109 cells was calculated and shown. d The anchorage-independent growth in KYSE150 and ECa109 cells was assessed by soft agar cloning assay. e The number of clones was counted and shown. The experimental data were representative of three independent experiments. Results were expressed as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001