Fig. 7.

MiR-133b inhibited tumor growth and lung metastasis of ESCC cells in mice.(a The photograph of the xenograft tumors formed by KYSE150 and ECa109 cells transfected with miR-133b agomir or miR-133b NC at 30 days after injection. b Tumor growth curves and c tumor weight of each groups were shown. d, e The level of miR-133b, and mRNA levels of EGFR, ITGB4, FAK, and Grb2 in the lung tissues of mice injected with KYSE150 or ECa109 cells were detected by RT-qPCR. f The protein levels of EGFR, ITGB4, p-FAK, FAK, and Grb2 in the lung tissues were assessed by western blotting. g The gray-scale value of the bands were quantitatively analyzed. The experimental data were representative of three independent experiments. Results were expressed as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001