Fig. 1.

Isolation of BJ-PLA₂-I from B. jararaca venom. a Chromatographic profile of Bothrops jararaca venom (200 mg) on Sephacryl S-200 column: Elution was carried out with 50 mM Ambic, pH 8.0. Inset, 12% SDS-PAGE of the fractions from A to G1 in non-reducing conditions. b Chromatographic profile of fraction F (~ 50 mg) on a Source 15Q column: Elution was carried out with 20 mM Tris-HCl, pH 8.0, and a linear concentration gradient of NaCl (from 0 to 1 M). Inset, 12% SDS-PAGE of the fraction of interest (arrow) in non-reducing conditions. c Chromatographic profile of fraction S.10 (~ 1.2 mg) on a Mono Q 5/50 GL column: Elution was carried out with 50 mM Ambic, pH 8.0, and a linear concentration gradient of NaCl (from 0 to 1 M). Inset, 12% SDS-PAGE of the fraction of interest (arrow) in non-reducing (1) and reducing (2) conditions. d Chromatographic profile of BJ-PLA₂-I (~ 200 μg) on a C18 reversed-phase column using a HPLC system: The column was previously equilibrated with solution A (0.1% TFA), and elution was performed at flow rate of 1 mL/min with a linear concentration gradient of solution B (70% acetonitrile and 0.1% TFA). Inset, 12% SDS-PAGE of BJ-PLA₂-I in non-reducing (1) or reducing (2) conditions