Fig. 6.

Inhibition of platelet aggregation by BJ-PLA₂-I. Initially, platelet-rich plasma (PRP) was incubated at 37 °C with ADP (5 μM) for 10 min to determine the percentage of platelet aggregation. Next, PRP was first incubated at 37 °C with BJ-PLA₂-I (20.5 μg/mL) for 5 min, followed by addition of ADP (5 μM) and evaluation for another 10 min to determine the inhibition promoted by the toxin. Results were expressed as mean percentages ± SD (n = 2) of two independent experiments. *p < 0.05 compared to the ADP group