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. 2018 Nov 22;8:36. doi: 10.1186/s13395-018-0182-x

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — The R16/17-Syn PDZ fusion protein restored sarcolemmal nNOSμ in Cmah/mdx and DBA/2 J-mdx mice. AAV viruses were injected into both TA muscles of three Cmah/mdx (n = 3) and three DBA/2 J-mdx mice (n = 3). Representative photomicrographs of serial muscle sections visualized for GFP, R17, syntrophin (Syn), nNOS expression, and nNOS activity staining. In non-transduced myofibers, there is no sarcolemmal nNOSμ restoration. Asterisk, the same myofiber in serial sections; square, the non-transduced myofiber. Scale bar = 50 μm