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. 2018 Nov 16;9:2613. doi: 10.3389/fimmu.2018.02613

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Copyright © 2018 Yang, Liu, Sun, Li, Tan, Yu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Single cell sorting of gp140 trimer specific memory B cell from PBMC of HIV infected patient. (A) gate on lymphocyte; (B) gate on IgM (PerCP-cy5.5) negative, 7AAD (PerCP-cy5.5) negative, CD3(PE-CY7) negative and CD14 (PE-CY7) negative population; the purpose is to remove IgM positive cells and T cells. (C) Gate on CD19 (FITC) positive and IgG (Pacific blue) positive population; the purpose is to gate the IgG positive B cells. (D) Gate on SF162gp140 trimer binder (PE), the purpose is to fish out antigen specific IgG positive B cells.