Fig. 2. Cellular uptake of TPP–Tat–Saporin–Alexa Fluor 488 liposomal conjugate in MC28 cells using laser scanning confocal microscopy. Cells were incubated with the conjugate for 24 h. Red colour in (A) represents the porphyrin fluorescence signal from TPP–Tat (excitation: 405 nm). Green colour in (B) represents the fluorescence signal from Saporin–Alexa Fluor 488 (excitation at 488 nm). C: Merged image of A and B; D: porphyrin fluorescence from empty TPP–Tat liposomes (excitation at 405 nm). E: control image of same field as D with empty TPP–Tat liposomes (excitation at 488 nm and detection at 510–560 nm). All images on same scale; scale bar: 10 μm.