Figure 1.

Expression of pancreatic duodenal homeobox protein-1 and neuroginin-3 during early development of islets cultured by direct contact with or without mesenchymes. Immunocytochemistry analysis of whole mount islets cells isolated from PPDL and SOC and cultured without (A-D) for PDL and I-L for SOC, or with (E-H) MSCs for PDL and M-P for SOC. Islet cells development was evaluated by anti-Pdx1 (green) and anti-Ngn3 (red). PDX1/NGN3 (orange). Nuclei were counter-stained in blue with DAPI. ), Scale bar = 100 µm