Figure 1.

Standard of care increases Myxoma virus (MYXV) spread.

Notes: (A) Timeline of treatment protocol for all experiments. (B) Cells were plated in replicates onto 12-well plates. Percent of GFP+ cells were analyzed by flow cytometry 12 hours after infection with vGFP and treatment as indicated in the protocol. Data represent summation of three independent experiments (P<0.05). (C) Cells were plated into replicates on to 12-well plates. The numbers of replication competent MYXV particles were determined by titering after harvesting from cells pretreated with the indicated treatment protocols in duplicate. Data represent the summation of two independent experiments. (D) Cells were plated onto 6-well plates in replicates. Images of GFP positive foci, infected with vGFP and treated as indicated, at 4× magnification 72 hours after infection under fluorescent microscopy. (E) Mean foci area was assessed at 24-hour intervals after infection with vGFP and treated as indicated for 72 hours. There was a significant difference between cells treated with 1 Gy of radiation and untreated cells (^*=P<0.011). Data are representative of three independent experiments.