The anti-tumor efficiency of unloaded MSCs-Exo, MSCs-Exo loaded with LNA-anti-miR negative control, and MSCs-Exo loaded with LNA-anti-miR-142-3p in female BALB/c mice bearing 4T1 or TUBO breast tumor after intravenous administration every 4 days.
Notes: (A) Average 4T1 tumor volume (i), survival analyses of 4T1 tumor-bearing mice (ii), and the actual weight of the dissected 4T1 tumors from each group on day 36 (iii). (B) Average tumor volume (i), survival analyses of TUBO tumor-bearing mice (ii), and the actual weight of the dissected TUBO tumors from each group on day 40 (iii) (n=5, mean ± SEM). In vivo delivery of LNA-anti-miR-142-3p via MSCs-Exo significantly reduced tumor growth rate (*P<0.05). The mice treated with MSCs-Exo loaded with LNA-anti-miR-142-3p had a significantly longer survival rate than PBS-treated mice or those treated with unloaded MSCs-Exo and MSCs-Exo loaded with LNA-anti-miR negative control (P<0.05).
Abbreviations: Exo + anti-miR-142-3p, exosomes loaded with LNA-anti-miR-142-3p; Exo + anti-miRNC, exosomes loaded with LNA-anti-miRnegative control; LNA, locked nucleic acid; MSCs-Exo, mesenchymal stem cells-derived exosomes; SEM, standard error of the mean.