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. 2018 Nov 20;13:7727–7747. doi: 10.2147/IJN.S182384

Figure 11.

Figure 11

Reduction of miR-142-3p and miR-150 levels and its effect on target genes by exosome-based delivery of LNA-anti-miR-142-3p in 4T1 and TUBO tumor tissues.

Notes: (A) qRT-PCR analysis showed significant reduction of miR-142-3p expression level (i) and significant increase of APC mRNA expression (ii) in both 4T1 and TUBO tumor tissues in mice injected with MSCs-Exo loaded with LNA-anti-miR-142-3p compared to three other groups, normalized to U6 snRNA. (B) Inhibition of miR-142-3p expression mediated by MSCs-Exo delivery of LNA-anti-miR-142-3p led to significant reduction of miR-150 expression level (i) and significant increase of P2X7R mRNA expression (ii) in both 4T1 and TUBO tumor tissues in mice injected with MSCs-Exo loaded with LNA-anti-miR-142-3p compared to three other groups, normalized to U6 snRNA. Data are presented as mean ± SEM (n=3) (*P<0.05).

Abbreviations: LNA, locked nucleic acid; MSCs-Exo, mesenchymal stem cells-derived exosomes; NCloaded exosomes, exosomes loaded with LNA-anti-miRnegative control; qRT-PCR, quantitative real-time PCR; SEM, standard error of the mean; snRNA, small nuclear ribonucleic acid.